个人简介

严明，副教授，硕士生导师。1996年于南京大学获生物化学理学硕士学位，1997-2000年在南京军区南京总医院检验中心工作，2001年至今在999全讯白菜网从事生物信息学与微生物应用技术的科研和教学工作，作为生物信息学研究室负责人，在蛋白质工程和代谢工程等领域的应用研究积累了丰富的经验。近年来参加了国家973项目“新一代生物催化与生物转化的科学基础”和“人工合成细胞工厂”等多项国家与省部级研究项目。在国内外检索期刊上发表论文30余篇，其中SCI论文10余篇，申请国家发明专利10余项，译有《比较基因组学手册：原理与方法》一书。

研究方向

1、酶的结构生物学与生物信息学

借助结构生物学和生物信息学技术深入研究重要工业酶的序列、结构和功能关系，在对酶蛋白三维空间结构进行解析的基础上，通过对酶蛋白进行构效解析、理性设计和定向进化，提出理性设计和定向进化策略来生产新型和改良工业酶。

2、精细化学品的生物催化与转化

开展工业生物催化剂及其催化过程的研究，采用酶或全细胞催化技术，生产化学法难以转化与合成的新型生物产品，或替代现行化学合成工艺中的复杂步骤。研究生物体及其酶进行定向物质转化的分子机制，开发新的生物合成技术。开发化工原料、医药原料、手性化合物、功能糖与糖基化学品等高值精细化学品的高效生物合成技术，以生物技术改造化学工业过程。

代表性成果

1.  Yang X, Lei G, Ying H, Xu L, Yan M: Enhancing the production of uridine 5'-monophosphate by recombinant Saccharomyces cerevisiae using a whole cell biocatalytic process. World Journal of Microbiology and Biotechnology 2011:1-7.

2. Liu H, Liu K, Yan M, Xu L, Ouyang P: gTME for Improved Adaptation of Saccharomyces cerevisiae to Corn Cob Acid Hydrolysate. Applied Biochemistry and Biotechnology 2011:1-10.

3. Ye Q, Li X, Yan M, Cao H, Xu L, Zhang Y, Chen Y, Xiong J, Ouyang P, Ying H: High-level production of heterologous proteins using untreated cane molasses and corn steep liquor in Escherichia coli medium. Applied microbiology and biotechnology 2010, 87(2):517-525.

4. Ye Q, Cao H, Zang G, Mi L, Yan M, Wang Y, Zhang Y, Li X, Li J, Xu L: Biocatalytic synthesis of (S)-4-chloro-3-hydroxybutanoate ethyl ester using a recombinant whole-cell catalyst. Applied microbiology and biotechnology 2010:1-9.

5. Ye Q, Cao H, Yan M, Cao F, Zhang Y, Li X, Xu L, Chen Y, Xiong J, Ouyang P: Construction and co-expression of a polycistronic plasmid encoding carbonyl reductase and glucose dehydrogenase for production of ethyl (S)-4-chloro-3-hydroxybutanoate. Bioresource technology 2010, 101(17):6761-6767.

6. Ye Q, Cao H, Mi L, Yan M, Wang Y, He Q, Li J, Xu L, Chen Y, Xiong J: Biosynthesis of (S)-4-chloro-3-hydroxybutanoate ethyl using Escherichia coli co-expressing a novel NADH-dependent carbonyl reductase and a glucose dehydrogenase. Bioresource technology 2010.

7.Yao T, Xu L, Ying H, Huang H, Yan M: The catalytic property of 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus on 3-hydroxypropionate. Applied Biochemistry and Biotechnology 2010, 160(3):694-703.

8. Yang X, Xu L, Yan M: Modeling metabolic response to changes of enzyme amount in yeast glycolysis. African Journal of Biotechnology 2010, 9(41):6894-6901.

9. Liu H, Yan M, Lai C, Xu L, Ouyang P: gTME for improved xylose fermentation of Saccharomyces cerevisiae. Applied Biochemistry and Biotechnology 2010, 160(2):574-582.

10. Hao N, Yan M, Zhou H, Liu H, Cai P, Ouyang P: The effect of AmtR on growth and amino acids production in Corynebacterium glutamicum. Applied Biochemistry and Microbiology 2010, 46(6):561-566.

11. Hao N, Li Y, Yan M, Ouyang PK: Prediction and analysis of the secreteomic in Corynebacterium glutamicum ATCC 13032. African Journal of Biotechnology 2010, 9(11):1561-1567.

12. Cao H, Mi L, Ye Q, Zang G, Yan M, Wang Y, Zhang Y, Li X, Xu L, Xiong J et al: Purification and characterization of a novel NADH-dependent carbonyl reductase from Pichia stipitis involved in biosynthesis of optically pure ethyl (S)-4-chloro-3-hydroxybutanoate. Bioresource technology 2010.

13. Ye Q, Yan M, Yao Z, Xu L, Cao H, Li Z, Chen Y, Li S, Bai J, Xiong J: A new member of the short-chain dehydrogenases/reductases superfamily: Purification, characterization and substrate specificity of a recombinant carbonyl reductase from Pichia stipitis. Bioresource technology 2009, 100(23):6022-6027.

14. Ye Q, Yan M, Xu L, Cao H, Li Z, Chen Y, Li S, Ying H: A novel carbonyl reductase from Pichia stipitis for the production of ethyl (S)-4-chloro-3-hydroxybutanoate. Biotechnology letters 2009, 31(4):537-542.

15. Xu W, Yan M, Xu L, Ding L, Ouyang P: Engineering the activity of thermophilic xylose isomerase by site-directed mutation at subunit interfaces. Enzyme and Microbial Technology 2009, 44(2):77-83.

16. Xu W, Cai P, Yan M, Xu L, Ouyang P: Molecular Dynamics Simulation of Temperature-dependent Flexibility of Thermophilic Xylose Isomerase. Chinese Journal of Chemical Physics 2009, 22:467.

17. XU W, CAI P, YAN M, XU L, OUYANG PK: Molecular docking of xylitol and xylose isomerase from Thermus thermophilus and model analysis. Chem J Chin Univ 2007, 28:971-973.

18. Li Y, Yan M, Wang K, Xu L, Xu W, Ouyang P: Study on dsRNA Mediated Gene Silencing in Saccharomyces cerevisiae: Suppressing the Expression of GRE3 Gene. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 2007, 34(3):292-298.